安徽农业科学2011，Vol.39Issue(13)：7857-7859,3.
人а干扰素转基因小鼠模型的建立
Establishment of Human Interferon-α Transgenic Mice Model
摘要
Abstract
[ Objective] To establish human interferon-α (IFN-α) transgenic mice, which can be used to study antiviral effect of IFN-α.[ Method] The expression vector was constructed by inserting the human IFN-α gene into a vector harboring CMV promoter. The transgenic mice were created by the method of microinjection. The genotype of transgenic lines was identified by PCR and southern blotting. [ Result] Four lines of transgenic mice of IFN-α were established. Lymphocytes of PCR-positive F1 generation mice were collected and identified by RTPCR, and mice of three lines were positive. ELISA and microplate dye-binding assay showed the expression of human IFN-α in the serum of mice which were positive in RT-PCR identification. [ Conclusion] The transgenic mice carrying CMV-controlled IFN-α gene has been estab lished successfully, which is essential for studying antiviral effect of IFN-α and genetic engineering breeding of disease-resistant animals.关键词
干扰素/转基因小鼠/CMV启动子Key words
Interferon/ Transgenic mouse/ Cytomegalovirus promoter分类
农业科技引用本文复制引用
李维纳,衣帅,刘烨,扈荣良,袁宝,任文陟,刘殿峰..人а干扰素转基因小鼠模型的建立[J].安徽农业科学,2011,39(13):7857-7859,3.基金项目
国家自然科学基金重大专项"抗口蹄疫、抗乳房炎转基因克隆牛的培育"(2009ZX08007-007B). （2009ZX08007-007B）
