热带作物学报2011，Vol.32Issue(1)：172-175,4.DOI:10.3969/j.issn.1000-2561.2011.01.034
采用反相高效液相色谱法同时测定杨梅果实中的有机酸
Determination of the Organic Acids from the Fruit of Myrica ruba by RP-HPLC
摘要
Abstract
A RP-HPLC method with a UV detector was established to determine the organic acids from the fruit of Myrica ruba.The chromatographic conditions were: Waters PhenomenexM C18(4.6 mmID×250 mm, 4 μm)used for the column(at 35 ℃)with 0.04mol·L-1 KH2PO4-H3PO4 buffer(pH2.4)as the mobile phase, and the flow rate 0.8 mL·min-1,detector wavelength UV-214 nm.The correlation coefficient of the calibration graphs was 0.999 0-0.999 9.The recovery was 88％～101％, RSD＜3.06％.关键词
反相高效液相色谱法/杨梅/有机酸Key words
High performance liquid chromatography/ Myrica ruba/ Organic acids分类
农业科技引用本文复制引用
刘文静,潘葳..采用反相高效液相色谱法同时测定杨梅果实中的有机酸[J].热带作物学报,2011,32(1):172-175,4.基金项目
福建省科技计划重点项目(2008N0031). （2008N0031）
