吉林农业大学学报2011，Vol.33Issue(2)：172-176,5.DOI:CNKI:22-1100/S.20110303.1506.00
玉米sbe2a基因RNAi载体的构建及转化玉米的初步研究
The Primary Study on Construction of RNAi Expression Vector of sbe2a Gene and Maize Transformation
摘要
Abstract
cDNA segment of corn starch branching enzymes gene sbe2 a was cloned by PCR technique and inserted into pMD18-T vector. The recombinant clone was detected by PCR and the sequence and fragments of restriction enzymes were analyzed. The results showed that the length of the clone sequence was 562 bp. The sense and and-sense fragments of clone sequence were inserted into downstream of 35S promoter of pCAMBIA1301 to construct over-expression RNAi vector. And then it was transformed into corn inbred lines“ TIE 7922” by Pollen-tube Pathway. It was confirmed primarily that the exogenous gene was integrated into corn genome via PCR analyzing the four transgenic plants. It could improve the content of amylase.关键词
玉米/淀粉分支酶基因sbe2a/RNAi/遗传转化Key words
maize/ starch branching enzyme gene sbe2a/ RNAi/ genetic transformation分类
生物科学引用本文复制引用
关淑艳,楚海娇,刘慧婧,刘广娜,刘学志,王丕武..玉米sbe2a基因RNAi载体的构建及转化玉米的初步研究[J].吉林农业大学学报,2011,33(2):172-176,5.基金项目
国家转基因专项(20082x08003-005),吉林省财政厅项目(200806),吉林省科技成果转化补助项目(20095044) （20082x08003-005）
