吉林农业大学学报2011，Vol.33Issue(2)：185-188,4.
小提质粒快速排除假阳性克隆的新方法
Rapid Mini-Preparation of Plasmid DNA for Eliminating False Positive Clones
摘要
Abstract
A rapid method was developed to greatly simplify the procedures of plasmid DNA purification within about 15 min and greatly reduce the experiment cost compared with traditional method. Seven recombinants were constructed and identified by PCR in order to confirm reliability of the new method. The results indicated that this new method was simple and inexpensive and suitable for gram-negative bacterium. It can be used for rapidly eliminating false positive clones.关键词
质粒/小量制备/假阳性克隆Key words
plasmid/ mini-preparation/ false postwe clone分类
生物科学引用本文复制引用
任大勇,李昌,秦艳青,杜寿文,诸晴丽,金宁一..小提质粒快速排除假阳性克隆的新方法[J].吉林农业大学学报,2011,33(2):185-188,4.基金项目
国家科技部重大传染病专项(2008ZX10004-015),解放军"十一五"科技攻关项目(06G127),吉林省高新技术产业发展项目(2010),长春市科技特派员行动计划项目(09KT04) （2008ZX10004-015）
