福建农林大学学报（自然科学版）2011，Vol.40Issue(2)：156-160,5.
水稻谷氨酸脱羧酶基因(OsGAD3)分子进化与表达分析
Molecular evolution and expression analysis of OsGAD3 gene from different rice varieties
摘要
Abstract
In this study, glutamate decarboxylase genes ( O.sC AD3s) were sucoessfully iuolated from 8 rice varieties by using molecular cloning techniques, then related bioinformatics and expression profiles were also analyzed. The results showed that there is low nucleotide sequence differentiation of less than 1％ among OsGAD3s from different rice varieties. The OsGAD3s all contain CaMbinding key amino acid sites on the C-terminal, which is similar to OsGADI but rather than OsGAD2. Phylogenic tree analysis indicated that different OsGAD3s can be divided into two subgroups of japonica andindica, which is consistent with conventional classification of rice. In addition, expression profile analysis indicated that OsGAD1, OsGAD2, OsGAD3, OsGAD4 and OsGAD5 were all expressed in the tested rice varieties with different levels, which may be associated with the varieties difference. The cloning and analysis of OsGADs in different rice varieties will lay a good foundation for further understanding of physiological roles of different GADs and breeding novel rice varieties rich in γ-aminobutyric acid (GABA) by metabolic engineering in the future.关键词
γ-氨基丁酸/水稻/谷氨酸脱羧酶/基因克隆/分子进化/表达分析Key words
γ-amino butyric acid/ rice/ glutamate decarbaxylase/ gene cloning/ molecular evolution/ expression analysis分类
生物科学引用本文复制引用
许辉,廖攀,许明,黄志伟,程祖锌,董彦君,开国银..水稻谷氨酸脱羧酶基因(OsGAD3)分子进化与表达分析[J].福建农林大学学报（自然科学版）,2011,40(2):156-160,5.基金项目
上海市科委项目(075405117,09QH1401900,08391911800) （075405117,09QH1401900,08391911800）
福建省粮食重大专项(2008NZ0001-4) （2008NZ0001-4）
上海市教委项目(09ZZ138,J50401) （09ZZ138,J50401）
国家转基因生物新品种培育科技重大专项(2009ZX08012-002B)和国家自然科学基金(30900110)资助. （2009ZX08012-002B）
