现代科学仪器Issue(2)：94-96,3.
光谱法研究异烟肼与牛血清白蛋白的相互作用
Spectroscopic Study on Interaction of Isoniazid with Bovine Serum Albumin
摘要
Abstract
The binding reaction of Isoniazid with bovine serum albumin was studied at 292K and 3 11K by fluorescence spectra.It was shown that Isoniazid has a strong ability of quenching the fluorescence of BSA, and the quenching mechanism of Isoniazid to BSA was a dynamic quenching.According to the Forster theory of non-radiation energy transfer, the binding distances (r) was 5.60 nm, the binding constants (Kb) were 5.32l×104 L·mol-1(292K), 3.189×104L·mol-1 (311K),respectively.The thermodynamic parameters ArHm=-20.343 kJ·mol-1,△rSm=20.8 J·mol-1 showed that the interaction of Isoniazid and BSA is mainly driven by electrostatic force.Synchronous spectrum was used to investigate the conformational changes of BSA.关键词
异烟肼/牛血清白蛋白/荧光光谱Key words
Isoniazid/ Bovine Serum Albumin (BSA)/ Fluorescence spectroscopy分类
化学化工引用本文复制引用
尚永辉,孙家娟,刘静..光谱法研究异烟肼与牛血清白蛋白的相互作用[J].现代科学仪器,2011,(2):94-96,3.基金项目
国家自然科学基金项目(20975081) （20975081）
陕西省教育厅科研计划项目(2010JK899) （2010JK899）
西北大学研究生交叉学科资助项目(07YJC09) （07YJC09）
