安徽农业科学2011，Vol.39Issue(13)：7729-7730,7733,3.
天然抑菌剂的提取工艺及抗芒果病原菌作用研究
Study on the Extraction Technology of Natural Anti-bacterial Agent and Its Antibacterial Activity to Mango Pathogen
摘要
Abstract
[ Objective ] The extracting technique of natural anti-bacterial agent resisting to mango mathogen from the natual medicine consisting of Flos Lonicerae, Flos Sophorae Immaturus and Herba Pogostemonis and its activity were researched. [ Method ] The concentration of ethanol and its amount, the number of extraction and its time in the technique were optimized through the orthogonal experiment and the lowest concentration of anti-bacterial agent in actual operation was tested by means of the plate diffusion method. [ Result ] The results showed that the best anti-bacteriostasis was available when the ratio of Flos Lonicerae, Flos Sophorae Immaturus and Herba Pogostemonis was 1: 1: 2. The optimal extracting condition was as follows: the reflux-extracting amount of 70％ alcohol was 6 times and the number if extraction was twice for 30 minutes/each. The lowest concentration of the inhibition to Colletotrichum gloeosporioides Penz and Phompsis sp was 6.25 mg/ml and to Botryodiplodia sp,12.50 mg/ml. [ Conclusion] The extracting technique was stable and feasibleand the extract from the mixture had a strong inhibition effect on typical mango pathogen.关键词
金银花/槐米/广藿香/芒果/抑菌成分/提取工艺Key words
Flos Lonicerae / Flos Sophorae Immaturus / Herba Pogostemonis/Mango/Extraction technology分类
农业科技引用本文复制引用
刘敬,王琼,赵斌..天然抑菌剂的提取工艺及抗芒果病原菌作用研究[J].安徽农业科学,2011,39(13):7729-7730,7733,3.基金项目
中山市农业科技攻关项目(20092A221) （20092A221）
中山火炬职业技术学院青年项目(2009Q014). （2009Q014）
