大连工业大学学报2011,Vol.30Issue(2):87-89,3.
真菌产人参皂苷葡萄糖苷酶基因的调取
Cloning of the ginsenoside glucosidase produced by fungus
摘要
Abstract
According to the N-terminal amino acid sequences of panaxsaponin-glucosidase produced by Absidia Sp. R84g, the complete sequence of GluGF was obtained using rapid-amplification of cDNA3' and cDNA5' ends, the RT-PCR. The length of complete sequence is 2149 bp, 3' UTR is 114 bp5'UTR is 82 bp, and the series of CDS length is 1923 bp.关键词
人参皂苷糖苷酶/3′RACE技术/5′RACE技术/RNA/PCRKey words
Panaxsaponin-glucosidase/ 5'RACE/ 3'RACE; RNA/ PCR分类
轻工纺织引用本文复制引用
杜枫,金凤燮,鱼红闪..真菌产人参皂苷葡萄糖苷酶基因的调取[J].大连工业大学学报,2011,30(2):87-89,3.基金项目
国家自然科学基金资助项目(30371744) (30371744)
辽宁省科学技术基金资助项目(20042132) (20042132)
辽宁省教育厅创新团队项目(2009T007). (2009T007)