烟台大学学报（自然科学与工程版）2011，Vol.24Issue(2)：116-120,5.
IL18-IL2融合基因的克隆及其在大肠埃氏菌(E.coli)中的表达
Cloning and Expression of a Fusion Protein IL18-IL2 in Escherichia Coli
摘要
Abstract
We report here the cloning and expressing of an fusion protein IL18 -IL2 containing the mature hu man interleukin-18 peptide and interleukin-2 peptide in Escherichia Coli. The mature human IL-I8 and IL-2 cDNAs are cloned by RT-PCR with the total RNAs, extracted from human PBMC stimulated by PHA, as tem plates. The fusion gene IL18 -IL2 connected by a flexible linker is inserted into the pBV220 expression vector and sequenced. Then the recombinant plasmid pBV-IL18 -IL2 is transformed into BL21 ( DE3 ) and induced at 42 ℃. SDS-PAGE analysis indicates that the relative molecular weight of IL18 -IL2 is about 34.5 ku and Western blot further confirms its correctness. The fusion protein IL18 -IL2 is successfully cloned and expressed in E. coli. This work has settled the foundation for further biological research on IL18 -IL2.关键词
白细胞介素-18/白细胞介素-2/pBV220载体/原核表达/融合蛋白Key words
interleukin-18/ interleukin-2 / pBV220 vector/ prokaryotic expression/ fusion protein分类
生物科学引用本文复制引用
杜广营,张国营,刘昆,栾金灵,段慧英..IL18-IL2融合基因的克隆及其在大肠埃氏菌(E.coli)中的表达[J].烟台大学学报（自然科学与工程版）,2011,24(2):116-120,5.基金项目
烟台大学青年基金资助项目(YX08Z3). （YX08Z3）
