烟台大学学报(自然科学与工程版)2011,Vol.24Issue(2):121-125,5.
中华补血草金属硫蛋白基因的克隆及高盐处理下的表达模式分析
Cloning and Analysis of Metallothionein Gene from Limonium sinense Kuntze
摘要
Abstract
The metallothionein gene, with an open reading frame of 249bp and encodes a protein of 82 amino acid residues, is cloned from Limonium sinense Kuntze. This protein has 14 Cys residues (Cys) and a con served sequence of C -C , C -X -C ,C -X -X -C at the end of both C -and N-terminal. There are two strong hydropho bic areas at 35 -48 and 52 - 68 amino acid residues. Expression analysis of MT by semi-quantitative-PCR technology showes the expression level is increasing gradually when Limonium sinense Kuntze is treated with 3% NaCI for 0,12,24,48 ,72 h. It reaches the highest levels at 48 h, then decreases. This study lays a foun dation for the deep research of MT from Limonium sinense Kuntze in response to salt stress.关键词
中华补血草/金属硫蛋白/基因克隆/表达模式/序列分析Key words
Limonium sinense Kuntze/ metallothioneins/ gene colne/ sequence analysis/ expression analysis分类
农业科技引用本文复制引用
刘瑜,陈世华,尹海波,李丽霞,赵吉强,张莹,韩会玲,郭善利..中华补血草金属硫蛋白基因的克隆及高盐处理下的表达模式分析[J].烟台大学学报(自然科学与工程版),2011,24(2):121-125,5.基金项目
山东省自然科学基金资助项目(Y2007D16) (Y2007D16)
国家自然科学基金资助项目(30870199) (30870199)
中国农业大学植物生理学与生物化学国家重点实验室开放课题基金资助项目(SKLPPBKF09008) (SKLPPBKF09008)
烟台大学博士基金资助项目(HY07B16). (HY07B16)