福建农林大学学报(自然科学版)2011,Vol.40Issue(2):122-127,6.
番木瓜胚性愈伤组织的诱导及体胚发生
Embryogenic callus induction and somatic embryogenesis of papaya
摘要
Abstract
This test regards immature embryos,leaves and cotyledons of papaya(Carica papaya L.)as explants,studying and exploring the effect of hormones, attachment, developing methods and conditions on the papaya embryogenic calli and somatic embryos induction.The experimental results showed that immature embryo was suit to somatic embryogennesis.The best medium for callus induction was modified MS+10 mg .L-1 2,4-D+5 mg .L-1 NAA+2 mg .L-1 KT+0.5 mg .L-1 BA,the most suitable medium for callus proliferation was modified MS+10 mg . L-1 2,4-D+2.5mg .L-1 NAA+1 mg .L-1 KT+0.5 mg . L-1 BA, and the best medium of somatic embryogenesis was modified MS + 10 mg . L-1 2,4-D +2 mg . L-1 KT. Embryogenic calli inducion from papaya coty1edon was difficult, calli induction from leaves or hypocotyl was easy, but somatic embryogenesis was very difficult.关键词
番木瓜/愈伤组织/体胚发生/幼胚Key words
papaya/ callus/ somatic embryogenesis/ immature zygotic embryo分类
农业科技引用本文复制引用
蔡雪玲,陈晓静,申艳红,何玮毅..番木瓜胚性愈伤组织的诱导及体胚发生[J].福建农林大学学报(自然科学版),2011,40(2):122-127,6.基金项目
福建省自然科学基金资助项目(2010J05048). (2010J05048)