草业学报2011，Vol.20Issue(2)：177-183,7.
马铃薯WRKY6基因的克隆、序列分析与原核表达研究
Cloning, sequence analysis and prokaryotic expression of the WRKY6 of potato
李立芹 1黄玉碧 1王西瑶1
作者信息
- 1. 四川农业大学农学院,四川,雅安,625014
- 折叠
摘要
Abstract
In higher plants, there are many WRKY transcription factors in WRKY family. They are widely involved in biotic stress; abiotic stress; growth; development and metabolism regulation. Cloning WRKY6 from potato by homology cloning. Analysis show that the WRKY6 belongs to the third group of WRKY family members, zinc-finger structure is C-X7-C-X23-H-X-C. Phylogeny results show WRKY6 is the most closest to AtWRKY70 with 58％ similarity, By SDS--PAGE analysis, the best expression conditions of WRKY6 are that host bacteria-Escherichia coli (DE3) is growing for 8 hours under 18℃ adding 0. 2 mmol/L IPTG (Isopropy1βD-thiogalactoside), purified protein of high specificity is obtained. This research lay. a solid foundation to further study the foundation of WRKY6.关键词
马铃薯/WRKY/克隆/序列分析/原核表达Key words
potato(Solanum tuberosum)/ WRKY gene/ cloning/ sequence analysis/ prokaryotic expression分类
农业科技引用本文复制引用
李立芹,黄玉碧,王西瑶..马铃薯WRKY6基因的克隆、序列分析与原核表达研究[J].草业学报,2011,20(2):177-183,7.
