中国药房2011，Vol.22Issue(19)：1740-1743,4.
康脑液2号对脑缺血再灌注大鼠VEGF、Ang-1与微血管密度的影响
Effects of Kangnao Liquid Ⅱ on VEGF, Ang-1 and MVD of Rats with Focal Cerebral Ischemia-reperfusion Injury
摘要
Abstract
OBJECTIVE: To investigate the influences of Kangnao liquid Ⅱ on vascular endothelial growth factor(VEGF), angiogenin-1 (Ang-1)and MVD in rats with focal cerebral ischemia reperfusion injury. METHODS: 180 healthy male SD rats were randomly divided into normal group, sham operation group, model group, Kangnao liquid Ⅱ high-dose, medium-dose and low-dose groups. Middle cerebral artery occlusion model was induced using suture method. Pathological changes were detected by HE staining and immunohistochemical method was used to investigate the expression of VEGF and Ang-1 in rats on 1 d, 3 d, 7 d,14 d, 21 d of reperfusion injury after ischemia for 2 h. Immunofluorescence labeling method was used to mark neovascular CD105 after 21 d of reperfusion and calculate MVD. RESULTS: Compared with model group, pathological changes of Kangnao liquid Ⅱ high-dose, medium-dose, low-dose groups was better than that of model group. The expressions of VEGF and Ang-1 increased at different time points significantly. MVD of cerebral infarction area was significant bigger than that of model group. CONCLUSION:Kangnao liquid Ⅱ can improve pathological change of rats with cerebral ischemia-reperfusion injury and protect nerve cells of cerebral infarction focus. It may be associated with Kangnao liquid Ⅱ can increase the expression of VEGF and Ang-1 to promote tissue angiogenesis.关键词
康脑液2号/脑缺血再灌注损伤/血管内皮生长因子/血管生成素-1/微血管密度Key words
Kangnao liquid Ⅱ/ Cerebral ischemia reperfusion injury/ Vascular endothelial growth factor/ Angiogenin-1/ MVD分类
医药卫生引用本文复制引用
梁起保,邹玉安,张晓丽,薛茜,石会娟,李瑞羽..康脑液2号对脑缺血再灌注大鼠VEGF、Ang-1与微血管密度的影响[J].中国药房,2011,22(19):1740-1743,4.基金项目
河北省医学科学研究重点课题(20090591) （20090591）
