中国药房2011，Vol.22Issue(19)：1790-1792,3.
HPLC法同时测定黄海棠中山柰酚和槲皮素的含量
Content Determination of Kaempferol and Quercetin in Hypericum ascyron by HPLC
摘要
Abstract
OBJECITVE: To establish the method for the content determination of kaempferol and quercetin in Hypericum ascyron. METHODS: HPLC method was adopted. The separation was performed on Purospher Star RP-C18(250 min×4.6 mm, 5 μm) column with mobile phase consisted of methanol-0.025 mol· L-1 phosphate (60∶40) at flow rate of 1.0 mL· min-1. The column temperature was 25℃ and determination wavelength was set at 370 nm. RESULTS: The linear ranges of kaempferol and quercetin was 0.018 4～0.128 8 μg(r=0.999 8) and 0.008～0.064 μg(r=0.999 9). The average recoveries were 97.12％ (RSD=1.03％ ,n=6) and 96.5 1％ (RSD = 1.32 ％, n =6). CONCLUSION: The method is sensitive, accurate, and reproducible for the simultaneous determination of kaempferol and quercetin in H. ascyron.关键词
黄海棠/高效液相色谱法/山柰酚/槲皮素/含量测定Key words
Hypericum ascyron/ HPLC/ Kaempferol/ Quercetin/ Content determination分类
医药卫生引用本文复制引用
张伟,于海林,宋艳丽,康文艺..HPLC法同时测定黄海棠中山柰酚和槲皮素的含量[J].中国药房,2011,22(19):1790-1792,3.基金项目
河南省科技厅重点攻关项目(102102310019) （102102310019）
