摘要
Abstract
OBJECTIVE: To establish the method for the content determination of cryptotanshinone, tanshinone Ⅰ, tanshinone Ⅱ A in Salviae miltiorrhizae from different habitats.METHODS: RP-HPLC method was adopted.The determination was performed on Kromasil TMC18(250 mm×4.6 mm, 5 μm)column with mobile phase consisted of methanol-water (75:25)at flow rate of 1 mL· min-1.The detection wavelength was set at 270 nm and the column temperature was 30 ℃.The injection size was 10 μL.RESULTS: The linear ranges of cryptotanshinone, tanshinone Ⅰ , tanshinone ⅡA in S.miltiorrhizae were 1.45～10.19 μg(r=0.999 8), 2.16～15.12 μg(r=0.999 6),3.15～22.06 μg(r=0.999 7), repsectively.The average recoveries were 99.44％, 99.48％, 99.34％, respectively.RSDs were 0.2 ％, 0.1％, 0.1％ (n = 6).There were significant differences in the contents of cryptotanshinone, tanshinone Ⅰ , tanshinone Ⅱ A in S.miltiorrhizae from different habitats.CONCLUSION: The method is stable, reliable and reproducible for the quality control of S.miltiorrhizae.关键词
丹参/隐丹参酮/丹参酮Ⅰ/丹参酮ⅡA/反相高效液相色谱法/产地/含量测定Key words
Salviae miltiorrhizae/ Cryptotanshinone/ Tanshinone Ⅰ/ Tanshinone ⅡA/ RP-HPLC/ Habitats/ Content determination分类
医药卫生
