西南林业大学学报2011，Vol.31Issue(2)：74-77,4.DOI:10.3969/j.issn.1003-7179.2011.02.017
响应面法优化β-葡萄糖苷酶均相水解的工艺条件
Study on Technology Optimization of Homogeneous Hydrolysis of β-glucosidase by Response Surface Methodology
摘要
Abstract
Box-Benhnken central composite design and Response Surface Methodology ( RSM) were applied to optimize the hydrolytic conditions from homogeneous hydrolysis of β-glucosidase by taking enzymatic saccharification of steam-exploded com stover as substrate , and temperature , pH value and P-glucosidase dosage as independent variables, while taking the glucose content in the homogeneously hydrolyzed liquor as dependent variable in the experiment. The results showed that the glucose content was significantly affected by temperature and β-glucosidase dosages. The optimum conditions for homogeneous hydrolysis of cellobiose enzymatic saccharification were concluded as : 52 ℃ as reaction temperature, pH value 4. 0, 1 IU/mL as β-glucosidase dosage. Under these conditions, the average glucose content reached 52. 8lmg/mL in the 3 verirication tests.关键词
β-葡萄糖苷酶/均相水解/纤维二糖溶液/响应面法Key words
β-glucosidase/ homogeneous hydrolysis/ cellobiose liquor/ Response surface methodology分类
化学化工引用本文复制引用
杨静,张晓萍,勇强,余世袁..响应面法优化β-葡萄糖苷酶均相水解的工艺条件[J].西南林业大学学报,2011,31(2):74-77,4.基金项目
国家自然科学基金项目(31070523)资助 （31070523）
国家林业局行业公益专项(200904017)资助 （200904017）
西南林业大学林产化学加工工程重点建设学科(XKX200907)资助. （XKX200907）
