水产学报2011，Vol.35Issue(4)：481-492,12.DOI:10.3724/SP.J.1231.2011.17149
三角帆蚌CAT基因cDNA全长克隆及表达分析
Cloning and expression analysis of CAT gene from Hypriopsis cumingii
摘要
Abstract
A 2 804 bp full-length cDNA sequence of catalase (CAT)gene from Hypriopsis cumingii was obtained by rapid amplification of cDNA ends.It consists of a 112 bp 5' UTR(untranslated region) ,a 1 388 bp ORF( open reading frame)and a 1 303 bp 3'UTR,and the deduced protein is composed of 462 amino acids,with calculated molecular weight of 52.7 ku, and its isoelectric point was 6.35.Motif analysis showed that CAT deduced amino acid sequence contained a highly conserved catalytic site motif “23FDRERIPERVVHAKGAG39".Twelve amino acids ( Aspl07, His153, Phe157, Ser160, Arg162, Asn172,Try174,Lys196,Va1261 ,Trp262, His264 and Try317 )of CAT gene of H.cumingii were identified as putative residues involved in NADPH binding, and they were different in different species.The CAT amino acid residues of H.cumingii shared a high similarity with other molluscs (99％), and shrimp, fish, amphibians,mammals(98％ -99％ ).The obtained CAT of H.cumingii was predicted as CAT3.NJ tree suggested that H.cumingii clustered with mollusca firstly, and then clustered with shrimp, fish, amphibians and mammals.Realtime quantitative RT-PCR results displayed that CAT gene was expressed in a wide range of seven organs, with the lowest level of transcripts found in kidney.Its expression was up-regulated in blood, which was significantly different from other organs.The expressions of other five organs were generally up-regulated first and then down-regulated.关键词
三角帆蚌/过氧化氢酶(CAT)/基因表达/RACE-PCRKey words
Hyriopsis cumingii/ catalase(CAT)/ gene expression/ RACE-PCR分类
生物科学引用本文复制引用
袁一鸣,李西雷,白志毅,汪桂玲,李家乐..三角帆蚌CAT基因cDNA全长克隆及表达分析[J].水产学报,2011,35(4):481-492,12.基金项目
国家"九七三"计划前期研究专项项目(2009CB126001) （2009CB126001）
国家自然科学基金项目(30871923) （30871923）
国家科技支撑计划项目(2006BAD01A13) （2006BAD01A13）
上海市科委地方院校能力建设项目(08390510100) （08390510100）
