水产学报2011，Vol.35Issue(4)：501-508,8.DOI:10.3724/SP.J.1231.2011.17204
刺参微卫星标记与生长性状体重、体长的相关分析
Correlation analysis of microsatellite DNA markers with growth traits of body weight and length in Apostichopus japonicus
摘要
Abstract
Ten microsatellite loci were selected to analyze the correlation with quantitative trait locus of body weight and length genomic of Apostichopus japonicus using single marker-based analysis. DNA of 60 eight months old individuals of Apostichopus japonicus which have differences in growth traits were analyzed by 10 microsatellite markers. The results showed that a total of 50 different alleles were found, the number of alleles in each locus was 3 to 7, and the number of mean valid alleles was 5. 0. The polymorphism information content(PIC) of ten microsatellite loci was 0.083 3 to 0.666 7, and the value of heterozygosity was 0. 236 1 to 0. 767 7, which proved the ten loci can be adopted to make correlation analysis. SPSS program was used to analyze the effects of these 10 microsatellites on body weight and length of Apostichopus japonicus. The results showed that AB, AD and CD genotypes of AjSSR02 had significant difference in body weight and length with other types, allele A of AjSSR04 played a positive role in both body weight and length, FF genotype of AjSSR06 had a significant impact on weight but not on length, and A and B allele of AjSSR09 played positive and negative roles in growth traits respectively.关键词
刺参/微卫星/遗传多样性/生长性状/相关分析Key words
Apostichopus japonicus/ microsatellite/ genetic diversity/ growth traits/ correlation analysis分类
生物科学引用本文复制引用
孙国华,杨建敏,孙孝德,宋志乐,王卫军,刘小静..刺参微卫星标记与生长性状体重、体长的相关分析[J].水产学报,2011,35(4):501-508,8.基金项目
山东省良种工程项目(2008-2011) （2008-2011）
山东省优秀中青年科学家奖励基金(博士基金)项目(2008BS06004) （博士基金）
山东省科技攻关项目(2008GG10005023) （2008GG10005023）
