信阳师范学院学报（自然科学版）2011，Vol.24Issue(2)：238-240,254,4.DOI:10.3969/j.issn.1003-0972.2011.02.023
甲基蓝共振瑞利散射法测定人免疫球蛋白
Resonance Rayleigh Scattering Spectrometric Determination of Human Immunoglobulin with Methyl Blue
摘要
Abstract
In BR buffer ( pH 4.2), human immunoglobulin ( HIgG) reacts with methyl blue to form ionassociation complexes, which greatly enhances the resonance Rayleigh scattering (RRS) intensity of the system at the wavelength of 410 nm.The optimum reaction conditions including pH, the concentration of methyl blue and the reaction time were investigated.Under optimum condition, the RRS intensity at 410 nm was linear to the concentration of HIgG in the range of 0.3 ～ 115.3 mg/L, and the detection limit was 0.1 mg/L.Based on this, a novel method for the sensitive determination of HIgG was developed.关键词
甲基蓝/共振瑞利散射/人免疫球蛋白Key words
methyl blue/resonance Rayleigh scattering/ human immunoglobulin分类
化学化工引用本文复制引用
黄克靖,孙俊永,井强山,吴莹莹..甲基蓝共振瑞利散射法测定人免疫球蛋白[J].信阳师范学院学报（自然科学版）,2011,24(2):238-240,254,4.基金项目
河南省高校科技创新人才基金项目(2010HASTIT025) （2010HASTIT025）
河南省科技创新人才资助项目(104100510020) （104100510020）
