高等学校化学学报2011，Vol.32Issue(4)：897-902,6.
低氧亲和力血红蛋白类氧载体的制备及大鼠动脉血压响应
Preparation of Hemoglobin-based Oxygen Carrier with Low Oxygen Affinity and Arterial Blood Pressure Responses in Rat
摘要
Abstract
Hemoglobin-based oxygen carriers (HBOCs) is a universally compatible, immediately available,oxygen-carrying resuscitative fluid being developed as a red cell substitute for use in urgent blood loss. The oxygen affinity is one of the most important parameters of HBOCs, and is not completely understood at present.It was reported that the low oxygen affinity is the main reason for vasoconstriction (hypertension) in transfusion. In order to study the relationship between the oxygen affinity and the hypertension, two different o-raffinose crosslinked hemoglobin-based oxygen carriers with low oxygen affinity, polymerized porcine hemoglobin (pPolyHb) and intra-molecular cross-linked porcine hemoglobin (pCLHb), were prepared and used to compare their effect on blood pressure in rat. The raffinose was first oxidized by sodium periodate (sodium periodate: raffinose= 6:1, molar ratio), yielding a completely oxidized and homogeneous polyaldehyde ring-opened raffinose( o-raffinose ), the cross-linker for polymerizing porcine deoxyhemoglobin. A low oxygen affinity (P50 =5.75 kPa) and mainly intra-molecular cross-linked 64000 hemoglobin (pCLHb) was produced when treated by o-raffinose for 1 h, and was further purified to yield a homogeneous 64000 pCLHb. When treated for 24 h, a high molecular weight(600000) with low oxygen affinity(p50 =7.87 kPa) polymerized hemoglobin (pPolyHb) was produced. These two products were then used for 50％ isovolemic exchange transfusion in rat.The results show that the pCLHb caused an immediate and sustained hypertension while the blood pressure of pPolyHb treated rat keeps normal, suggesting that hypertension was related to the 64000 hemoglobin and low oxygen affinity wasn't the main cause of hypertension.关键词
猪血红蛋白/棉籽糖/血红蛋白氧载体/氧亲和力/高血压Key words
Porcine hemoglobin/ Raffinose/ Hemoglobin-based oxygen carrier/ Oxygen affinity/ Hypertension分类
化学化工引用本文复制引用
严坤平,惠文利,罗超,谢于斗,彭明丽,陈超..低氧亲和力血红蛋白类氧载体的制备及大鼠动脉血压响应[J].高等学校化学学报,2011,32(4):897-902,6.基金项目
国家"八六三"计划项目(批准号:2006AA02A143)和陕西省教育厅专项科研计划项目(批准号:09JK784)资助. （批准号:2006AA02A143）
