摘要
Abstract
[Objective] The research aimed to study the optimum liquid fermentation condition of Trichoderma hamatum Thl2 strain, which provided the theory basis for studying and developing the biological control Trichoderma agent. [ Method ] By using the diluted soil plate method and the plate confrontation method, Trichoderma hamatum Thl2 strain was identified. Moreover, the influences of carbon source, nitrogen source, fermentation time, fermentation temperature, initial pH, inoculation bacteria amount, bottling capacity and shaker speed on Trichoderma mycelium yield were studied. [ Result] The optimum liquid fermentation conditions of Trichoderma Thl2 strain were: the glucose as the carbon source, peptone as the nitrogen source, fermentation temperature was 25 ℃, initial pH was 6, inoculation bacteria amount was 0.8 ml, bottling capacity was 50 ml, shaker speed was 180 r/min. Trichoderma hamatum Thl2 strain was cultivated 3 d under the condition, and the mycelium yield was the highest. [ Conclusion ] Under the optimum liquid cultivation condition, Trichoderma hamatum Thl2 strain could provide the mycelium amount which was used for preventing and controlling the grassland Fusarium oxysporum and Rhizoctonia solani to the utmost extent.关键词
木霉/液体发酵/生物防治Key words
Trichoderma/ Liquid fermentation/ Biological control分类
农业科技
