安徽农业科学2011，Vol.39Issue(13)：7582-7584,7946,4.
黑麦特异性PCR反应体系的建立
Establishment and Optimization of Rye-specific PCR Reaction System
摘要
Abstract
[ Objective ] The aim was to establish the optimal rye-specific PCR reaction system for rye. [ Method ] The ordinary wheat "Chinese Spring", S165, rye, octoploid triticale and hexaploid triticale were used as materials to carry out study on the effect of the amount of template DNA, primers, dNTPs, Mg2+ concentrations, Taq DNA polymerase and annealing temperature on the rye-specific PCR reaction system of rye. [ Result ] The genomic DNA extracted by modified CTAB DNA extraction method showed high quality, which was satisfied for the PCR reaction template. The rye-specific PCR reaction system was 25 μl, including 10 × buffer solution, 1. 5 mmol/L MgC12,200 μmol/L dNTP,40 ng primers, 40 - 60 ng DNA template and 1 U Taq DNA polymerase. [ Conclusion ] The optimal rye-specific PCR reaction system was established, which provided basis for the identification of exogenous germplasm of rye in wheat background.关键词
黑麦/PCR反应体系/优化Key words
Rye/ PCR reaction system/ Optimization分类
农业科技引用本文复制引用
任如意..黑麦特异性PCR反应体系的建立[J].安徽农业科学,2011,39(13):7582-7584,7946,4.基金项目
黑龙江省普通高校青年学术骨干计划项目(1152G047) （1152G047）
牡丹江师范学院大学生创新项目. （）
