安徽农业科学2011，Vol.39Issue(13)：7592,7777,2.
大豆DDRT分析中单引物造成假阳性的研究
False Positives Caused by Single Primer in DDRT Analysis of Soybean
魏益凡 1魏先运2
作者信息
- 1. 吉林农业大学农学院,吉林长春130118
- 2. 通化市高等职业技术学院,吉林通化134001
- 折叠
摘要
Abstract
[ Objective] The aim was to explore the reasons of false positives in Different Display Reverse Transcription (DDRT) analysis.[ Method] Soybean varieties “Jilin 30” and “Tongnong 13” were used as materials to carry out analysis on false positives in DDRT analysis.[ Result] An important origin of false positives appeared in DDRT analysis was the non-specific amplification caused by the combination of single primer and cDNA. The parallel PCR test of single primer should be set so as to verify whether the obtained fragments were the false positives or the PCR productions combined with single primer. [ Conclusion] This study had provided basis for improving the success rate of DDRT experiment.关键词
mRNA差异显示技术/单引物扩增/假阳性/应用Key words
Different Display Reverse Transcription (DDRT)/ Single primer PCR/ False positive/ Application分类
农业科技引用本文复制引用
魏益凡,魏先运..大豆DDRT分析中单引物造成假阳性的研究[J].安徽农业科学,2011,39(13):7592,7777,2.
