里氏木霉外源表达载体的构建OA
Establishment of Trichoderma reesei Construct for Expressing External Genes
[目的]构建里氏木霉外源表达栽体.[方法]以里氏木霉40359的CBHI启动子和终止子构建了里氏木霉40359外源表达栽体,并用该表达载体成功表达了抗潮霉素B磷酸转移酶基因,得到6株可在含175 mg/L潮霉素的基础培养基上生长的抗性菌株;提取6株抗性菌株的DNA整合到里氏木霉菌株中,并对其进行潮霉素耐受性检测.[结果]6株抗性菌株的抗潮霉素能力比原始菌株提高了75%;转基因菌株的潮霉素抗性可以稳定遗传,证明表达栽体构建成功.[结论]为开展里氏木…查看全部>>
[ Objecfve ] The study was to construct a Trichoderma reesei vector for expressing external genes. [ Method ] Using CBHI promoter and terminator of T. reesei strain 40359, we constructed an expression vector of T. reesei strain 40359 for expressing Hpt gene and got 6 strains capable of growing on basic medium containing 175 mg/L hygromycin B, further conducted hygromycin resistance test. [ Result] In comparison with the original strain( wild type), hygromyci…查看全部>>
张晓烜 ;王傲雪
东北农业大学成栋学院,黑龙江哈尔滨150030东北农业大学生命科学学院,黑龙江哈尔滨150030
农业科技
里氏木霉载体遗传转化
Trichoderma reesei Vector Genetic transformation
《安徽农业科学》 2011 (13)
7617-7620,4
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