安徽农业科学2011，Vol.39Issue(13)：7617-7620,4.
里氏木霉外源表达载体的构建
Establishment of Trichoderma reesei Construct for Expressing External Genes
张晓烜
1王傲雪2
作者信息
- 1. 东北农业大学成栋学院,黑龙江哈尔滨150030
- 2. 东北农业大学生命科学学院,黑龙江哈尔滨150030
- 折叠
摘要
Abstract
[ Objecfve ] The study was to construct a Trichoderma reesei vector for expressing external genes. [ Method ] Using CBHI promoter and terminator of T. reesei strain 40359, we constructed an expression vector of T. reesei strain 40359 for expressing Hpt gene and got 6 strains capable of growing on basic medium containing 175 mg/L hygromycin B, further conducted hygromycin resistance test. [ Result] In comparison with the original strain( wild type), hygromycin resistance the 6 engineered strains was increased by 75％; the hygromycin resistance could inherit stably. [ Conclusion ] Our results laid basis for biological study on T. reesei at molecular and genetically engineering levels.关键词
里氏木霉/载体/遗传转化Key words
Trichoderma reesei/ Vector/ Genetic transformation分类
农业科技引用本文复制引用
张晓烜
,王傲雪..里氏木霉外源表达载体的构建[J].安徽农业科学,2011,39(13):7617-7620,4.
