传感技术学报2011，Vol.24Issue(4)：467-474,8.DOI:10.3969/j.issn.1004-1699.2011.04.001
阵列型微拓扑结构基底的制备及其对神经细胞电生物物理特性的影响
Fabrication of Arrayed Topographic Substrates and Their Effects on the Electrobiophysical Properties of Neuronal Cells*
摘要
Abstract
UV lithography, silicon etching and soft lithography were adopted to fabricate micropillar arrayed cell culture substrates. With this approach, poly(dimethylsiloxane) (PDMS)topographic substrates with pillar featuring dimensions equal to or larger than 4 μm could be susccessfully fabricated, with a pillar height of 4 μm. The fabrication of substrates with a pillar feafuring dimension of 2 μm might represent the maximal fabrication capability of this method, as pillar lodging or missing could be found. With a simple tilting angle method, we fabricated a topographic substrate formed with a packed polystyrene microbead array. The arrayed beads could be as small as 2 μm in diameter or even in submicrometer range. Cell culture experiments showed that arrayed topographical substrates can pro mote adhesion or spreading, affecting resting membrane potential establishement and enhance the responsiveness of voltage-gated calcium channels of neuronal cells. The arrayed topographic substrates provide an efficient tool for engineering the microenvironments for sensor neuronal cells.关键词
细胞生物传感器/功能响应性/阵列型拓扑结构基底/软光刻/倾斜角法/电压依从式钙通道/静息膜电位Key words
cell-based biosensor/ functional responsiveness/ arrayed topographic substrate/ soft lithography/ tilting/angle method/voltage-gated/calcium/channel/resting/membrane/potential分类
医药卫生引用本文复制引用
吴泽志,朱满根,Kisaalita W S,Wang L,Zhao Y P,林雨,黄岂平,廖彦剑,Li C Z..阵列型微拓扑结构基底的制备及其对神经细胞电生物物理特性的影响[J].传感技术学报,2011,24(4):467-474,8.基金项目
重庆市科技攻关重点项目(CSTC2009AB5202) （CSTC2009AB5202）
国家自然科学基金项目(30870570) （30870570）
重庆大学"211工程"三期创新人才培养计划建设项目(S-09104) （S-09104）
