山东农业大学学报（自然科学版）2011，Vol.42Issue(2)：238-242,5.
多胎与单胎山羊发情期卵巢褪黑素受体基因的克隆测序分析
SEQUENCING ANALYSIS OF MELATONIN RECEPTOR IN OVARY OF GOAT
摘要
Abstract
In order to clarify the genetic mechanisms of melatonin affected goat reproduction traits, differential display PCR, cloning and sequencing techniques were used to detected the expressions of melatonin receptors (MelR1a) in ovary tissure of Jining Gray Goat and Yimeng Black Goat in oestrus stage, while MelR1a genes from the two kinds of goats were cloning and sequencing.The results showed that MelR1 a genes were expressed in ovary tissues of the both goats, and the bases sequences of MelR1 a genes were the same.The results suggest that MelR1 a genes expressed in both goat ovaries play an important role in controlling the reproduction traits obviously, and make clear that Mella is conservative.关键词
济宁青山羊/沂蒙黑山羊/卵巢/褪黑素受体-1a/差异显示PCRKey words
Jining Grey Goat/ Yimeng Black Goat/ ovary tissue/ melatonin receptor-1a/ DDRT-PCR分类
农业科技引用本文复制引用
汪运舟,曾启繁,陈维云,王慧,王树迎..多胎与单胎山羊发情期卵巢褪黑素受体基因的克隆测序分析[J].山东农业大学学报（自然科学版）,2011,42(2):238-242,5.基金项目
国家大学生创新基金(SRT:081043411)、国家自然科学基金(30671498)和山东省科技攻关(2006GG2209004)项目 （SRT:081043411）
