新疆农业科学2011,Vol.48Issue(3):522-527,6.
石河子地区棉花黄萎病菌培养特性和致病力分化研究
Study on the Culture Characteristic and Pathogenic Differentiation of Veticillium dahliae from Cotton in Shihhotze
摘要
Abstract
[ Objective] This research focused on the pathogenic differentiation of Veticdlium dahliae from Cotton in Shihezi to reveal the reason of disease explosion on pathogenic aspect. [Method]29 representative strains were collected and observed by culture characteristic and determined by pathogenicity type base on differential host test, PCR assay and the defoliate symptoms. [ Result] The colony of strains differed in growth rates, and these sclerotium and filament type were domain, 75.9% and 17.2% separately. The results of inoculating differential host test showed that strong, medium and weak pathogenicity strains occupied 51.7% , 31 .0% and 17 .3% of total examined strains, respectively. However the defoliating and non - defoliating strains occupied 37 .9% and 58.6% of them, and the corresponding average index was 39 .5 and 27 .6, respectively. Defoliating symptoms was observed in plants inoculated hoth defoliating and non - defoliating strains, but severity have manifest difference. Thus, the defoliating symptom is not only related with the pathogenicity and pathogenicity type of pathogens, but also with the resistance of cotton cultivars . [ Conclusion]The sclerotium type strains are the majority in Shihezi. The pathogenicity of defoliating strains is obvious stronger than that of non - defoliating strains, and the explosion of the strong pathogenicity and defoliating population is one of the key factor of disease increasing visibly in filed.关键词
棉花/黄萎病菌/培养特性/致病力分化/落叶症状/石河子地区Key words
cotton/ Verticillium dahliae/ culture charactenstic/ pathogenicity diffrerentiation/ symptoms of defoliation / Shihhotze分类
农业科技引用本文复制引用
韩宏伟,任毓忠,高峰,刘培源,吉丽丽,李春,李国英..石河子地区棉花黄萎病菌培养特性和致病力分化研究[J].新疆农业科学,2011,48(3):522-527,6.基金项目
农业部基础性工作专项(SB2007FY027) (SB2007FY027)