中国医科大学学报2011，Vol.40Issue(3)：193-195,3.DOI:CNKI:21-1227/R.20110328.1512.001
无镁诱导培养大鼠海马神经元癫痫放电模型中延迟整流钾电流的变化
Changes in Delayed Rectifier K+ Current in Hippocampal Neurons Cultured in Mg《'2+》-free Medium in Rat Model of Epileptiform Discharge
摘要
Abstract
Objective To detect the changes in delayed rectifier K+ current in hippocampal neurons cultured in Mg, 2+-free medium in rat model of epileptiform discharge. Methods The hippocampal tissue was sampled from l-day-old Wistar rats and used for the primary culture of hippocmpla neurons. After 12 to 16 days of primary culture,the neurons were treated with Mg2+-free extracellular fluid for 3 hours,and then the discharge activity and delayed rectifier K + current were recorded by patch clamp. Results After treated with Mg2+-free extracellular fluid,the neurons showed spontaneous “epileptiform discharge”,and the delayed rectifier K+ current significantly increased. Conclusion The increase of delayed rectifier K + current might be related to the underlying mechanism of spontaneous epileptiform discharge in hippocampal neurons cultured in Mg2+-free medium.关键词
海马/膜片钳/癫痫放电/延迟整流钾电流Key words
hippocampus/ patch clamp/ epileptiform discharge/ delayed rectifier K+ current分类
医药卫生引用本文复制引用
郭凤,孙威,姚阳,高青华,闵冬雨,封瑞,胡慧媛,蔡际群,郝丽英..无镁诱导培养大鼠海马神经元癫痫放电模型中延迟整流钾电流的变化[J].中国医科大学学报,2011,40(3):193-195,3.基金项目
国家自然科学基金资助项目(81001429,31071004,30670761) （81001429,31071004,30670761）
