色谱2011，Vol.29Issue(4)：314-319,6.DOI:10.3724/SP.J.1123.2011.00314
液相色谱-质谱联用系统在肝硬化不同阶段代谢轮廓研究中的应用
Application of liquid chromatography-mass spectrometry in the study of metabolic profiling of cirrhosis in different grades
杜振华 1张磊 2刘树业2
作者信息
- 1. 天津医科大学三中心临床学院,天津300070
- 2. 天津市第三中心医院,天津市人工细胞重点实验室,天津300170
- 折叠
摘要
Abstract
The metabolic profiles were obtained by high performance liquid chromatography combined with a LTQ Orbitrap XL mass spectrometer (HPLC-LTQ Orbitrap XL MS) platform to analyze serum specimens from the people of healthy control group and patients of hepatitis B virus (HBV)-induced cirrhosis. Then the data were analyzed with the pattern recognition methods and nonparametric test. The orthogonal partial least squares-discriminant analysis (OPLSDA) mode (R2(Y) =90. 1％, Q2 = 66. 7％) was constructed by the serum metabolic profiles of Child-Pugh grades A, B, C groups and a healthy control group in the training set and the good discrimination ability of this mode for the testing set was demonstrated with the accuracy of 93.8％. The corresponding specific metabolic biomarkers used to distinguish different cirrhosis grades were discovered, such as lysophosphatidylcholine (LPC), glycolchenodeoxycholic acid (GCDCA), cysteine, glycine, aminoadipic acid, pipecolic acid. The results suggest that the metabolic profiling of serum can be used to construct the discrimination mode and discover the metabolic biomarkers for the HBV-induced cirrhosis, which will support the diagnosis and evaluation of the HBV-induced cirrhosis.关键词
液相色谱-质谱联用/正交偏最小二乘判别分析/乙肝肝硬化/代谢轮廓/代谢组学/血清Key words
liquid chromatography-mass spectrometry (LC-MS)/ orthogonal partial least squares-discriminant analysis (OPLS-DA)/ hepatitis B virus (HBV)-induced cirrhosis/ metabolic profile/ metabolomics/ serum分类
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杜振华,张磊,刘树业..液相色谱-质谱联用系统在肝硬化不同阶段代谢轮廓研究中的应用[J].色谱,2011,29(4):314-319,6.
