色谱2011，Vol.29Issue(4)：330-334,5.DOI:10.3724/SP.J.1123.2011.00330
氨基酸消耗谱在区分不同作用机制抗癌药物中的应用
Classification of anticancer drugs with different mechanisms based on amino-acid consumption profiling in culture media
摘要
Abstract
An approach for quantitative determination of amino-acid consumption profiling in culture media by high performance liquid chromatography with fluorescence detection ( HPLCFLD) was developed and validated, using o-phthalic dicarboxaldehyde (OPA)as the derivatizing reagent and norvaline as the internal standard. Mobile phase A was l0 mmol/L Na2HPO4Na2B4O7 buffer (pH 7.95 ), and mobile phase B was acetonitrile-methanol-water (45: 45: 10, v/ v/v). The linear elution program was 5％ B at the start and 52％ B at the end in 35 min. The 17 free amino-acids (FAAs) were separated satisfactorily in 33 min. Following HeLa cells incubation in conditioned medias of taxol (4 μmol/L) and mitomycin ( 75 μmol/L), respectively, with control for 24 h, the media 17 amino-acid consumption profilings were determined, and then analyzed by multivariate statistical analysis based on Matlab7. 1 software platform. Relation analysis performed by partial least squares-discriminant analysis (PLS-DA) indicated that in comparison with the control group, the media amino-acid consumption profiling can distinguish the two anticancer drugs with different mechanisms, which provides a new perspective for the pre-classification of drug action mechanisms during the screening of new anticancer drugs. Meanwhile, the idea from the outer into the inner has convenient and economic characteristics.关键词
高效液相色谱/氨基酸消耗谱/紫杉醇/丝裂霉素/HeLa细胞/代谢组学Key words
high performance liquid chromatography (HPLC)/ amino-acid consumption profiling/ taxol/ mitomycin/ HeLa cell/ metabolomics分类
化学化工引用本文复制引用
韩晓菲,玉龙星,杨乾栩,肖红斌..氨基酸消耗谱在区分不同作用机制抗癌药物中的应用[J].色谱,2011,29(4):330-334,5.基金项目
国家自然科学基金项目(No.20705033). （No.20705033）
