动物营养学报2011，Vol.23Issue(4)：670-676,7.DOI:10.3969/j.issn.1006-267x.2011.04.019
复合抗氧化剂对脂多糖诱导的大鼠肠道损伤的修复作用
The Use Composite Antioxidants to Repair Intestinal Tract Injuries of Rats Induced by lipopolysaccharide
摘要
Abstract
This experiment adopted rats as experimental animals to build a model of intestinal tract injuries induced by lipopolysaccharide (LPS) and to study the use of composite antioxidants on intestinal reparation.Thirty six SD rats at 42 days of age were randomly assigned to 3 groups with 12 rats in each group. The control and induction group were fed with the basal diet while the reparation group was fed with diet adding composite anfioxidants in it. On day 5, 9, 13 and 17, rats in induction and reparation group were intrapefitoneally injected 0.8 mg LPS per kg of body weight while rats in control group were injected the same amount of saline. On day 22 of experiment, 6 rats in each group were randomly selected and received D-xylose by gavage to determine the absorption of intestinal tract. The results showed as follows: 1 ) compared with the control group, in induction group, the average daily gain (ADG) of rats was decreased and the concentration of malondialdehyde (MDA) was increased significantly ( P ＜ 0.05 ), the activities of superoxide dismutase (SOD)and glutathione peroxidase (GSH-Px) were decreased significantly (P ＜0. 05 ), the liver coefficient was decreased by 11.13％ (P ＜0.05), D-( + )-xylose concentration was decreased by 72.72％ (P ＜0.05), and the activity of maltase was decreased by 65.74％ (P ＜0.05) in induction group. 2) Compared with the inducfion group, the concentration of MDA was decreased by 49.31％ (P ＜0. 05 ), the activities of SOD and GSH-Px in liver were increased by 40.96％ and 35.04％ (P ＜0. 05), the liver coefficient, D-( + )-xylose concentration and the activity of maltase were increased significantly ( P ＜ 0.05 ), and the crypt depth was increased significantly ( P ＜ 0. 05) in reparation group. In conclusion, the composite anfioxidants can repair the intestinal tract injuries induced by LPS and enhance the function of intestinal tract. [ Chinese Journal of Animal Nutrition ,2011,23 ( 4 ) :670-676 ]关键词
复合抗氧化剂/LPS/肠道/自由基代谢/大鼠Key words
composite antioxidants/ LPS/ intestinal tract/ free radical metabolism/ rats分类
农业科技引用本文复制引用
赵珂立,徐建雄,陈小连,王啸春..复合抗氧化剂对脂多糖诱导的大鼠肠道损伤的修复作用[J].动物营养学报,2011,23(4):670-676,7.基金项目
国家自然科学基金项目(30972103) （30972103）
