动物营养学报2011，Vol.23Issue(4)：677-685,9.DOI:10.3969/j.issn.1006-267x.2011.04.020
米曲霉固体发酵降解豆粕植酸及其生长条件的优化
Solid-state Fermentation with Aspergillus oryzae Using Soybean Meal as a Substrate: Degradation of Phytate and Optimization of Fungal Biomass Growth
摘要
Abstract
The aim of the present study was to optimize the solid-state fermentation with Aspergillus oryzae to degrade phytic acid and increase Aspergillus oryzae biomass growth in soybean meal, and to investigate the correlation between phytic degradation and fungal biomass growth. The response surface methodology by Box-Be-hnken design was used to optimize the ratio of substrate to water, inoculum size and duration. A total of 17 runs including 5 fermentation conditions and 5 replicates at the center point were set in this experiment. Each fermentation condition consisted of 3 levels ( the ratios of substrate to water were 0.5, 1.0 and 1.5, inoculum sizes were 4％, 8％ and 12％, and durations were 72, 96 and 120 h). Ergosterol was used as a tool to estimate Aspergillus oryzae biomass. The results showed that the minimum phytic acid content was 2.38 mg/g dry matter (DM) when the ratio of substrate to water was 0.5, inoculum size was 4％ and fermentation duration was 120 h. The maximum ergosterol content was 396.74 mg/kg DM when the ratio of substrate to water was 0. 5, inoculum size was 12％ and duration was 120 h. In additoin, a significant negative correlation between phytic acid degradation and fungal biomass growth of substrate in solid-state fermentation was detected. In conclusion, the optimal conditions for phytic acid degration can be achieved when the ratio of substrate to water is 0.5, inoculum size is 4％ and duration is 120 h, and the optimal conditions for Aspergillus oryzae growth can be achieved when the ratio of substrate to water is 0.5, inoculum size is 12％ and duration is 120 h. Aspergillus oryzae is of high efficiency in phytic acid degradation, and shows a potential use in industry-scale solid-state fermentation. Ergosterol can be a good indicator for phytase generation or phyfic acid degradation in solid-state fermentation of soyban meal.关键词
响应面法/固体发酵/米曲霉/植酸/麦角固醇Key words
response surface methodology/ solid-state fermentation/ Aspergillus oryzae/ phytic acid/ ergosterol分类
农业科技引用本文复制引用
高有领,汪财生,朱秋华,钱国英..米曲霉固体发酵降解豆粕植酸及其生长条件的优化[J].动物营养学报,2011,23(4):677-685,9.基金项目
Project from the Science and Technology Department of Zhejiang province (2009C12239) （2009C12239）
project from the Science and Technology Bureau of Ningbo City (#2006C100093) （#2006C100093）
modem microbiology technology and application,subject of priority among priorities in universities of Zhejiang Province （）
