棉花学报2011，Vol.23Issue(2)：147-153,7.
棉花枯萎病菌AFLP分子标记体系的建立及初步应用
Establishment and Application of AFLP Analysis System for Fusarium oxysporum f.sp.vasinfectum
摘要
Abstract
An amplified fragment length polymorphism(AFLP) analysis system was constructed by using HaeⅢ/Pst Ⅰ digested DNA to analyze different isolates of Fusarium oxysporum f.sp.vasinfectum.Three primer combinations were selected from 32primer pairs and employed in the optimized AFLP system.Three reference strains representing physiological races 3, 7 and 8 of F.oxysporum f.sp.vasinfectum from China were characterized by the optimized AFLP system.Cluster analysis using data generated by AFLP makers showed the system could clearly separate all three F.oxysporum f.sp.vasinfectum races.AFLP analysis of 20 isolates ofF.oxysporum f.sp.vasinfectum from four provinces categorized all isolates into the group of race 7 in high level of Dice similarity coefficients(0.96), and the genetic diversities were observed among different isolates in the same physiological group.关键词
棉花枯萎病菌/AFLP/分子标记/优化/遗传多样性Key words
Fusarium oxysporum f.sp.vasinfectum/ AFLP/ molecular marker/ optimization/ genetic diversity分类
农业科技引用本文复制引用
王晓光,郭庆港,么奕清,李社增,鹿秀云,李宝庆,马平..棉花枯萎病菌AFLP分子标记体系的建立及初步应用[J].棉花学报,2011,23(2):147-153,7.基金项目
现代农业产业技术体系专项资金 （）
