安徽农业科学2011,Vol.39Issue(11):6450-6452,3.
拮抗真菌菌株BOS-013对稻瘟病菌的抑制作用
Inhibition Effect of Antagonistic Fungal Strain BOS-013 against the Pathogen of Rice Blast ( Pyricularia oryzae)
摘要
Abstract
[ Objective ] To search for the beneficial microorganisms and their metabolic products with control effect against rice blast, the paper studied the inhibition effect of the fermentation broth of actinomycetes BOS-013 strain against the pathogen of rice blast (Pyricularia oryzae). [ Method] With the pathogen of rice blast as the target indicator fungus, the inhibition effect of the fermentation broth of BOS-013 strain and its extracts from different solvents against the pathogen were determined using cylinder plate method and filter paper method. [ Resuit] The results showed that the inhibition rate of the fermentation broth of BOS-013 strain with fungus amount of 4 × l08 cfu/ml against the pathogen of rice blast was 92.0% ± 0.5%; the fermentation broth still had inhibition effect against the pathogen of rice blast after inactivation under high temperature and high pressure, and the inhibition rate was 60.0% ± 0.5%. After absorbed by non-polar CAD-45 type macroporous adsorption resin and eluted by 60% ethanol, the antifungal activity of the fermentation broth was still high, and the diameter of inhibition zone reached 33.0 mm. [ Conclusion] The fermentation broth of BOS-013 strain had significant inhibition effect against the pathogen of rice blast.The results would provide foundation for further clarification of inhibition mechanism of BOS-013 strain fermentation broth against the pathogen of rice blast and its practical application.关键词
放线菌/拮抗/水稻稻瘟病/抑制率Key words
Actinomycete/ Antagonism/ Rice blast/ Inhibition rate分类
农业科技引用本文复制引用
吕跃东,王勇,张文革,谢程程,宫立晶,王雪..拮抗真菌菌株BOS-013对稻瘟病菌的抑制作用[J].安徽农业科学,2011,39(11):6450-6452,3.基金项目
鞍山市科技计划项目(2006SH19). (2006SH19)