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拮抗真菌菌株BOS-013对稻瘟病菌的抑制作用

吕跃东 王勇 张文革 谢程程 宫立晶 王雪

安徽农业科学2011,Vol.39Issue(11):6450-6452,3.
安徽农业科学2011,Vol.39Issue(11):6450-6452,3.

拮抗真菌菌株BOS-013对稻瘟病菌的抑制作用

Inhibition Effect of Antagonistic Fungal Strain BOS-013 against the Pathogen of Rice Blast ( Pyricularia oryzae)

吕跃东 1王勇 1张文革 2谢程程 1宫立晶 1王雪1

作者信息

  • 1. 辽宁科技大学化工学院,辽宁鞍山114044
  • 2. 鞍山师范学院附属卫生学校,辽宁鞍山114001
  • 折叠

摘要

Abstract

[ Objective ] To search for the beneficial microorganisms and their metabolic products with control effect against rice blast, the paper studied the inhibition effect of the fermentation broth of actinomycetes BOS-013 strain against the pathogen of rice blast (Pyricularia oryzae). [ Method] With the pathogen of rice blast as the target indicator fungus, the inhibition effect of the fermentation broth of BOS-013 strain and its extracts from different solvents against the pathogen were determined using cylinder plate method and filter paper method. [ Resuit] The results showed that the inhibition rate of the fermentation broth of BOS-013 strain with fungus amount of 4 × l08 cfu/ml against the pathogen of rice blast was 92.0% ± 0.5%; the fermentation broth still had inhibition effect against the pathogen of rice blast after inactivation under high temperature and high pressure, and the inhibition rate was 60.0% ± 0.5%. After absorbed by non-polar CAD-45 type macroporous adsorption resin and eluted by 60% ethanol, the antifungal activity of the fermentation broth was still high, and the diameter of inhibition zone reached 33.0 mm. [ Conclusion] The fermentation broth of BOS-013 strain had significant inhibition effect against the pathogen of rice blast.The results would provide foundation for further clarification of inhibition mechanism of BOS-013 strain fermentation broth against the pathogen of rice blast and its practical application.

关键词

放线菌/拮抗/水稻稻瘟病/抑制率

Key words

Actinomycete/ Antagonism/ Rice blast/ Inhibition rate

分类

农业科技

引用本文复制引用

吕跃东,王勇,张文革,谢程程,宫立晶,王雪..拮抗真菌菌株BOS-013对稻瘟病菌的抑制作用[J].安徽农业科学,2011,39(11):6450-6452,3.

基金项目

鞍山市科技计划项目(2006SH19). (2006SH19)

安徽农业科学

0517-6611

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