安徽农业科学2011，Vol.39Issue(11)：6543-6544,2.
美味牛肝菌合成漆酶的发酵条件研究
Study on Synthesis Conditions of Laccase from Boletus edulis
摘要
Abstract
[ Objective ] The aim was to study the optimum fermentation conditions for the synthesis of laccase from Boletus edulis. [ Method ]By using the submerged culture method, the effect of different carbon source, nitrogen source, pH and temperature on the laccase production from Boletus edulis. [ Result] The optimum synthesis conditions of laccase from Boletus edulis were as follows: glucose 2.0％, beef extract 0.5％, KH2PO4 0.4％, MgSO4 7H2O 0.3％, trace element solution 8％, Vitamin BI 0. 003％, pH 4.5, temperature 28 ℃ and culture time 8 d. [ Conclusion] Under these conditions, the maximum laccase production was 71.27 U/ml, the study provided scientific basis for the development and application of laccase from Boletus edulis.关键词
美味牛肝菌/漆酶/液体深层培养Key words
Boletus edulis/ Synthesis of laccase/ Submerged culture分类
农业科技引用本文复制引用
陈今朝,王剑锋,王慧超,谭永忠..美味牛肝菌合成漆酶的发酵条件研究[J].安徽农业科学,2011,39(11):6543-6544,2.基金项目
重庆市教委科研项目(KJ101304). （KJ101304）
