色谱2011，Vol.29Issue(5)：389-393,5.DOI:10.3724/SP.J.1123.2011.00389
超高效液相色谱-质谱法比较黑顺片单煎液及其复方煎煮液中次乌头碱在大鼠体内的吸收
Comparison of intracorporal absorption of hypaconitine in Heishunpian decoction and its compound recipe decoction by ultra performance liquid chromatography-quadrupole time- of-flight mass spectrometry
摘要
Abstract
An ultra performance liquid chromatography-quadrupole time-of-flight mass spectrometry (UPLC/Q-TOF MS) method was developed for the determination of hypaconitine in the plasma of the rats administered with Heishunpian (HSP) decoction, Zhufu (ZF) decoction and Gancaofuzi (GF) decoction, separately.Hypaconitine and the internal standard were separated on a ZORBAX Extend-C18 column.The concentration-time curve of hypaconitine in the plasma was protracted, and pharmacokinetic parameters were calculated, then the pharmacokinetic comparison of hypaconitine between HSP, ZF and GF was carried out for the first time.The results of the methodological test showed that the plasma concentration of hypaconitine presented good linear relationship in the range of 0.02 - 10 ng/mL.The results of the sample determination showed that the absorptive degree of hypaconitine from ZF decoction in the plasma was lower than that from HSP decoction, while the absorptive degree of hypaconitine from GF decoction in plasma was higher than that from HSP decoction.It was deduced that some components in ZF decoction can restrain the absorption of hypaconitine in plasma, and some components in GF decoction may promote the absorption of hypaconitine in plasma, and the difference in drug effects between ZF decoction and GF decoction may derive from the different absorptive degrees of hypaconitine in plasma.The pharmacokinetic property of hypaconitine was proposed to be non-linear dynamics on the basis of the drug elimination half life ( T1/2 ) and the area under the plasma concentration-time curve (AUC).关键词
超高效液相色谱-四极杆飞行时间质谱/次乌头碱/黑顺片/复方煎煮液/药物动力学参数Key words
ultra performance liquid chromatography-quadrupole time-of-flight mass spectrometry ( UPLC/Q-TOF MS)/ hypaconitine/ Heishunpian/ compound recipe decoction/ pharmacokinetic parameter分类
化学化工引用本文复制引用
辛杨,皮子凤,宋凤瑞,刘志强,刘淑莹..超高效液相色谱-质谱法比较黑顺片单煎液及其复方煎煮液中次乌头碱在大鼠体内的吸收[J].色谱,2011,29(5):389-393,5.基金项目
国家"973"中医理论专项基金项目(No.2011CB505305)和国家自然科学基金项目(Nos.81073040,30472134). （No.2011CB505305）
