色谱2011，Vol.29Issue(5)：454-457,4.DOI:10.3724/SP.J.1123.2011.00454
高效液相色谱-串联质谱法测定化妆品中的欧前胡素和异欧前胡素
Determination of imperatorin and isoimperatorin in Cosmetics by high performance liquid chromatography-tandem mass spectrometry
摘要
Abstract
A novel method for the simultaneous determination of imperatorin and isoimperatorin in cosmetics by high performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) was developed. The imperatorin and isoimperatorin were extracted by methanol. The analytes were separated on a C18 column using 10 mmol/L ammonium acetate/ methanol-5 mmol/L ammonium acetate aqueous solution (80:20, v/v) as mobile phase, and then detected by HPLC-MS/MS using multiple reaction monitoring ( MRM ) mode. The good linearities (r ＞ 0. 999 ) were achieved for the target compounds over the range of 0. 25 -20 μg/L. The limits of quantification (LOQs) were 0. 5 mg/kg. The recoveries for the imperatorin. and isoimperatorin varied from 82.2％ to 105％ and 80. 0％ to 103％ with the relative standard deviations ranging from 2. 7％ to 4. 9％ and 1.8％ to 4.6％ respectively in the range of 0. 5010.0 mg/kg. The method can meet the requirements for the simultaneous determination of imperatorin and isoimperatorin in cosmetics.关键词
高效液相色谱-串联质谱/欧前胡素/异欧前胡素/化妆品Key words
high performance liquid chromatography-tandem mass spectrometry (HPLC-MS/ MS)/ imperatorin/ isoimperatorin/ cosmetics分类
化学化工引用本文复制引用
陈捷,徐娟,熊莉华..高效液相色谱-串联质谱法测定化妆品中的欧前胡素和异欧前胡素[J].色谱,2011,29(5):454-457,4.基金项目
检验检疫行业标准制定计划(No.2009B093). （No.2009B093）
