中国生物防治学报2011，Vol.27Issue(1)：55-62,8.
取食转Bt基因水稻褐飞虱对杀虫剂的敏感性及其代谢酶的活性
Insecticide Susceptibility and Activity of Metabolic Enzymes of Brown Planthopper,Nilaparvata lugens(St(a)l)(Hemiptera: Deiphacidae)Feeding on Transgenic Bt Rice
摘要
Abstract
The toxicities of five commonly-used insecticides against different populations of rice brown planthopper, Nilaparvata lugens, fed on transgenic rice with crylAb gene, KMD1 and KMD2 for different generations were measured and compared with those on non-transgenic parental rice variety, Xiushui 11.Activities of detoxification enzymes (EST and GST), target enzyme (AChE), and protective enzymes (SOD, CAT and POD) from different populations of N.lugens fed on KMD1, KMD2, and Xiushui 11 and the content of Cry protein were also detected.For all five insecticides tested,there were no significant differences in LD50 values among different populations of N.lugens fed on KMD1 KMD2, and Xiushui 11 continuously in the laboratory and collected from the rice fields.For all six metabolic enzymes tested, there was no significant difference of enzyme activities among different populations of N.lugens fed on KMD1, KMD2 and Xiushui 11 for 1 and 9 generations in the laboratory or collected from the rice fields.The content of Cry protein was relatively stable in different populations of N.lugens fed on Bt rice, and was higher in N.lugens fed on KMD2.It was concluded that transgenic rice lines with cry gene, KMD1 and KMD2, had no significant effects on the susceptibility to insecticides and activities of metabolic enzymes of N.lugens.关键词
转基因水稻/褐飞虱/杀虫剂敏感性/代谢酶Key words
transgenic rice/ Nilaparvata lugens/ insecticide susceptibility/ metabolic enzymes分类
农业科技引用本文复制引用
李兆亮,姚洪渭,陈洋,田俊策,彭于发,叶恭银..取食转Bt基因水稻褐飞虱对杀虫剂的敏感性及其代谢酶的活性[J].中国生物防治学报,2011,27(1):55-62,8.基金项目
国家重大基础研究发展计划(973)(2007CB109202) （973）
国家转基因生物培育重大专项(2008ZX08011-01) （2008ZX08011-01）
