中国生物防治学报2011,Vol.27Issue(1):82-87,6.
红树内生细菌菌株Kc-38的抗菌物质及对采后芒果炭疽病的防效
Antibiotic Substances Produced by Mangrove Endophytic Bacteria Kc-38 and Biocontrol Efficacy on Anthracnose of Postharvest Mangoes
摘要
Abstract
The endophytic bacterial strain Kc-38 isolated from the tissue of Kandelia candel had strong inhibitory activity against many plant pathogenic fungi, such as Colletotrichum gloeosporioides, Fusarium oxysporum, and so on.According to the morphological, physiological and biochemical characteristics, and 16S rDNA sequence analysis, strain Kc-38 was identified as Bacillus amyloliquefaciens.The strain effectively suppressed mango anthracnose disease caused by C.gloeosporioides.When tested on postharvest mango fruits and treated with the bacterial suspension and its cellular exudates, the control efficacy on the 9th d reached 65.12 % and 62.79 %, respectively.Further studies showed that the antibiotic substances produced by the strain were some kind of antifungal proteins.The proteins were demonstrated to be UV-tolerant, thermostable and inhibitory at pH value ranged from 2.0 to 11.0, but more inhibitory at pH 2.0 - 8.0 than at pH9.0- 11.0.关键词
内生细菌/芒果炭疽病/抗菌物质/生物防治Key words
endophytic bacteria/ mango anthracnose disease/ antibiotic substance/ biocontrol分类
农业科技引用本文复制引用
汪远,詹儒林,何红,吕柱彬,余小琴,梁永琪,陈鸿宇..红树内生细菌菌株Kc-38的抗菌物质及对采后芒果炭疽病的防效[J].中国生物防治学报,2011,27(1):82-87,6.基金项目
广东省科技计划(2009B020310015,2009B030803055) (2009B020310015,2009B030803055)