肿瘤防治研究2011，Vol.38Issue(6)：628-631,4.DOI:10.3971/j.issn.1000-8578.2011.06.005
文殊兰叶氯仿提取物诱导NCI-H460细胞凋亡的研究
Study on Apoptosis of NCI-H460 Cells Induced by Chloroform Extract of Leaves from Crinum Asiaticum
摘要
Abstract
Objective To investigate the effect of chloroform extracts (CE) of leaves from Crinum asiaticum on proliferation and apoptosis of non-small cell lung cancer(NSCLC) NCI-H460 cells. Methods The activity of cell growth inhibition of NCI-H460 cells was measured by MTT assay. Morphological changes of cell nuclei were observed by Hoechst 33258 staining. The expression levels of Bcl-2 and Bax proteins were examined by immunocytochemistry staining. Flow cytometry was performed to measure the cell cycle. Results CE displayed growth inhibitory activity against NCI-H460 cells with IC50 values of 36. 22 ±3. 04, 41.21 ± 2. 50 and 62. 55 ± 3.47 mg/L for 24,48 and 72 h, respectively. The effect enhanced with an increase in the concentration of CE. The condensed chromatin, nuclear fragmentation and a large number of apoptotic bodies was observed by Hoechst 33258 staining. The percentage of apoptosis in NCI-H460 cells increased after treated with CE, compared with control. The expression level of Bax protein in NCI-H460 cells cultured with CE were higher than that of control, and the expression level of Bcl2 protein in NCI-H460 cells dealt with CE were lower than that of control. The arrest of NCI-H460 cells in G1/S phases was induced by CE. Conclusion In vitro CE significantly inhibited the growth of NCIH460 cells. Antitumor mechanism of NCI-H460 cells may be associated with the changes of cell cycle and cell apoptosis.关键词
非小细胞肺癌/NCI-H460/文殊兰叶/细胞凋亡Key words
NSCLC/ NCI-H460/ Leaves from Crinum asiaticum/ Cell apoptosis分类
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陈建荣,杨扬,杨月..文殊兰叶氯仿提取物诱导NCI-H460细胞凋亡的研究 [J].肿瘤防治研究,2011,38(6):628-631,4.
