广东医学2011，Vol.32Issue(15)：1945-1949,5.
柯萨奇-腺病毒受体不同胞内功能域缺失突变载体的构建与表达
Construction and expression of different intracellular functional-domain deleted recombinant vector of coxsackie-adenovirus receptor
摘要
Abstract
Objective To construct different intracellular functional - domain deleted recombinants of Coxsackie -adenovirus receptor ( CAR ) for functional analysis. Methods Recombinant pcDNA3. 1/V5/His - ΔCK2 , pcDNA3. 1/V5/His - ΔPKC, pcDNA3. 1/V5/His - ΔTYR and pTOPO - tailless were constructed amplified on the basis of three -step deletion mutation PCR. The recombinants were transfected into ovarian cancer cell line SKOV3, in which low CAR was expressed, by Lipofectamine 2000. Their protein expression levels were detected by western blot. Results All the mutated sequences were consistent with our envisagement confirmed by DNA sequencing and restriction analysis. Furthermore, CAR expression was up - regulated in SKOV3 transfected with recombinants. Conclusion Recombinants provide important groundwork in the study of molecular mechanisms of different functional domains of CAR in ovarian cancers.关键词
柯萨奇-腺病毒受体/功能域缺失突变重组体/卵巢癌Key words
Coxsackie - adenovirus receptor/ functional domain deleted recombinants/ ovarian cancer引用本文复制引用
范良生,王鸿雁,王蓓蓓,陈刚..柯萨奇-腺病毒受体不同胞内功能域缺失突变载体的构建与表达[J].广东医学,2011,32(15):1945-1949,5.基金项目
国家自然科学基金资助项目(编号:30700895,30672227,30770913),科技部"973"计划资助项目(编号:2009CB521808) （编号:30700895,30672227,30770913）
