中国生物防治学报2011，Vol.27Issue(1)：88-92,5.
花生青枯病内生拮抗细菌的鉴定、抗菌活性及其田间防效
Identification,Antimicrobial Activity and Field Control Efficacy of an Endophytic Bacteria Strain against Peanut Bacterial Wilt
摘要
Abstract
An endophytic bacteria strain BZ6-1, isolated from the stem of healthy peanut plants in Ralstonia solanacearum-infested fields, suppressed R. solanacearum greatly and was of broad spectrum against plant pathogenic fungi. According to its morphology and 16S rDNA sequence, the strain BZ6-1 was preliminarily identified as Bacillus amyloliquefaciens. Diameter of inhibition zone reached 34.0 mm under the optimal fermentation conditions for antibiotic production, which was molasses 10 g, peptone 0.5 g and yeast extract 0.5 g in lL medium at pH 8.5, temperature 25 ℃, rotation rate 200 r· min- 1, medium amount 50 mL in 250 mL flask and incubation period 21 h. Field trials demonstrated that the control efficiency of strain BZ6-1 against peanut bacterial wilt disease was 62.3％.关键词
花生青枯病/解淀粉芽孢杆菌/内生细菌/抗菌活性/田间防效Key words
peanut bacterial wilt/ Bacillus amyloliquefaciens/ endophytic bacteria/ antimicrobial activity/ field control efficacy分类
农业科技引用本文复制引用
王小兵,骆永明,刘五星,李振高..花生青枯病内生拮抗细菌的鉴定、抗菌活性及其田间防效[J].中国生物防治学报,2011,27(1):88-92,5.基金项目
国家自然科学基金重点项目(40432005) （40432005）
中国科学院知识创新工程重要方向项目(CXTD-Z2005-4) （CXTD-Z2005-4）
