中药材2011，Vol.34Issue(5)：665-669,5.
外源NO供体硝普钠(SNP)对盐胁迫下紫苏种子萌发及幼苗抗氧化酶活性的影响
Effect of Exogenous Nitric Oxide Donor SNP on Seed Germination and Antioxidase Activities of Perilla frutescens Seedlings Under NaCl Stress
摘要
Abstract
Objective :To explore the method of improving the salt resistance ability of seeds and seedlings of Perilla frutescens under NaCl stress.Methods:Physiological indexes of Perilla frutescens seeds treated by sodium nitroprusside(SNP) under NaCl stress like the germination vigor, germination rate, germination index and vigor index were measured.And other indexes like the activities of nitrate reductase(NR) ,malondialdehyde(MDA) ,superoxide(SOD) ,peroxidase(POD) and catalase(CAT) were also measured.Results: The germination indexes of Perilla frutescens seeds under NaCl stress had obvious inhibition.But after the treatment with different concentrations of SNP,every germination indexes were all increasecL And the seeds that treated with SNP with the concentration of 0.1 mmol/L has the most significantly increase in every index.The germination vigor was 60.4％ ,the germination rate was 78.3％ ,the germination index and vigor index were 13.7 and 0.1093 respectively.The content of MDA was decreased after the treatment of SNP.The activities of four enzymes included NR,SOD,POD and CAT were increased with the treatment of SNP,and get the maximin( 1.52 μg/g · h 、0.32 U/mg、5.9 U/mg and 4.8 U/mg respectively)with the concentration of 0.1 mmol/L SNP.Conclusion:SNP with concentration of 0.1 mmol/L could significantly alleviate the damages to the seeds and seedlings of Perillafrutescens under NaCI stress,and promote the salt resistance of the seeds and seedlings.关键词
紫苏/一氧化氮/盐胁迫/种子萌发/抗氧化酶/生理特性Key words
Perilla frutescens (L.) Britt./ Nitric Oxide/ Salt stress/ Seed germination/ Antioxidase/ Physiological characteristics分类
农业科技引用本文复制引用
张春平,何平,杜丹丹,韦品祥,喻泽莉,谢英赞,刘海英..外源NO供体硝普钠(SNP)对盐胁迫下紫苏种子萌发及幼苗抗氧化酶活性的影响[J].中药材,2011,34(5):665-669,5.基金项目
国家自然科学基金资助项目(30070080) （30070080）
