波谱学杂志2011，Vol.28Issue(2)：177-189,13.
Nanodisc体系在膜蛋白结构与功能研究中的应用
The Nanodics: A Novel Tool to Study Membrane Protein Structure and Function
摘要
Abstract
Membrane proteins are responsible for a wide range of essential physiological processes, and important drug targets for treating diseases. However, structural study on membrane proteins lags far behind that on soluble proteins, and this, in large part,is due to technical difficulties in preparing homogeneous, stable and structurally relevant samples in a membrane-like environment. Phospholipid bilayer Nanodisc is novel model membrane derived from high-density lipoprotein particles, and has proven to be useful in structural studies on membrane proteins. It has been shown that Nanodisc can provide a “native-like” lipid bilayer environment for many membrane proteins, such as VDAC-1 (voltage-dependent anion channel), GPCRs (G-protein-coupled receptors) and cytochrome P450s, thus enabling structural studies on these membrane proteins with NMR/surface plasmon resonance (SPR) techniques. Furthermore, with more sophisticated techniques such as modification of membrane scaffold proteins (MSP), the use of Nanodisc may yield structural and functional insights into a wide variety of membrane proteins in a biologically relevant membrane environment.关键词
液体核磁共振(NMR)/膜蛋白/Nanodisc/高密度脂蛋白Key words
NMR/ membrane protein/ Nanodisc/ high-density lipoprotein分类
数理科学引用本文复制引用
毕允晨,王玉娟,王俊峰..Nanodisc体系在膜蛋白结构与功能研究中的应用[J].波谱学杂志,2011,28(2):177-189,13.基金项目
中国科学院百人计划资助项目(1600000292-1BQ),国家自然科学基金资助项目(31070665). （1600000292-1BQ）
