实用临床医药杂志2011，Vol.15Issue(7)：16-20,5.
整合素连接激酶对阿霉素诱导心肌细胞凋亡的影响
Effects of integrin-linked kinase on apoptosis induced by doxorubicin in cardiomyocytes
摘要
Abstract
Objective To explore the effects of integrin -linked kinase(ILK) on apoptosisinduced by doxorubicin (DOX) in cardiomyocytes and to investigate its possible mechanism. Methods Cultured neonatal rat cardiomyocytes were randomly divided into three groups: control group, GFP + DOX group and ILK + DOX group. The apoptosis rate of myocardial cells was determined by terminal deoxynucleotidyl transferase- mediated dUTP- biotin nick end labeling (TUNEL). Expression of several apoptosis -related genes, including Fas, FasL, Bax and Bc1-2 in cardiomyocytes was examined by fluorescent quantitative real - time PCR. Results The ratio of apoptotic cardiomyocytes in GFP + DOX group was significantly higher than that in control group( P ＜ 0.01 ), but in ILK + DOX group, it was lower than that in GFP + DOX group(P ＜0.05). Compared with control group, the mRNA expression of Fas, FasL and Bax was higher(P ＜0.01 ), and the mRNA expression of Bcl -2 was lower than that in control group ( P ＜ 0.01 ). In ILK + DOX group, the mRNA expression of Fas, FasL and Bax was lower than that in GFP + DOX group ( P ＜ 0.05 ), and the mRNA expression of Bcl - 2 was higher( P ＜ 0.05 ). Conclusion Over - expression of ILK inhibits DOX - induced apoptosis in cardiomyocytes by up - regulating the expression of Bcl - 2 and inhibiting the expression of Fas, FasL and Bax.关键词
整合素连接激酶/阿霉素/心肌细胞/凋亡Key words
Integrin - linked kinase/ Doxorubicin/ cardiomyocyte/ apoptosis分类
医药卫生引用本文复制引用
孙静娴,白剑,王丙剑,毛雅晶,徐标..整合素连接激酶对阿霉素诱导心肌细胞凋亡的影响[J].实用临床医药杂志,2011,15(7):16-20,5.基金项目
国家自然科学基金项目(81070195) （81070195）
