中药材2011，Vol.34Issue(5)：738-746,9.
IEC-6细胞迁移药理实验模型建立的研究
Studies on Cell Migration Model in Intestinal Epithelial Restitution for Pharmacological Research
摘要
Abstract
To set up a suitable IEC-6 migration model for pharmacological research and observe the effect of complex polysaccharide from Astragalus membranaceus(Fisch.) Bge.var.mongholicus(Bge.) Hsiao to IEC-6 cell migration. Methods:The main conditions related to the establishment of the model,including the planting density of the cell,the observation time after scratching,the oncentration of the auxiliary material Matrigel,the treatment of the serum-starvation,the concentration of a-difluoromethylornithine (DFMO),an inhibitor of the cell migration,were investigated respectively;and the effects of the tested medicines on the model were observed. Results:4×105 cell/mL was the suitable planting density of the cell in the 6-well plate;at the 24th hour after scratching was the appropriate time to count the migrating cells; and the proper concentration of Matrigel was 5% ;the serum-starvation could evidently reduce the migrating cells, so the culture medium should contain the serum;2.5~5 mmol/L DFMO was proper for inhibition of the cell migration.Complex polysaccharide from Astragalus membranaceus and spermidine both can promote cell migration. Conclusion:The established model of IEC-6 cell migration was suitable for intestinal epithelial restitution such as the researches on pathophysiological mechanisms is the effects of the medicines on the cell migration.关键词
IEC-6/胃肠黏膜损伤/细胞迁移Key words
IEC-6/Gastrointestinal mucosa injury/Cell migration分类
医药卫生引用本文复制引用
胡灿,李茹柳,卢文彪,羊燕群,王静,陈蔚文..IEC-6细胞迁移药理实验模型建立的研究[J].中药材,2011,34(5):738-746,9.基金项目
国家自然科学基金(30772753) （30772753）
广东省自然科学基金(7300343) （7300343）
上海市教育委员会E-研究院建设计划项目(E03008) （E03008）
