中药材2011，Vol.34Issue(5)：746-750,5.
蛇床子素对腰椎间盘突出致坐骨神经痛大鼠的作用及其机制研究
Effects and Mechanisms of Osthole on Sciatica Induced by Lumber Disc Herniation
摘要
Abstract
Objective: To Study the effects of osthole on sciatica induced by lumber disc herniation and its mechanisms. Methods:54 male SD rats were randomly divided into 6 groups. Model(M) group ( n = 12) :Autologous nucleus pulposus was harvested from the tail and applied to the L5 dorsal root ganglion (DRG) and epidural space. Epidural catheterization was performed. Control(C) group (n = 12) :On the basis of nucleus pulposus group,50 μL tween-80 was administered epidurally on the day 6th after surgery. T2(n =6), T6 ( n = 12 ), T13 ( n = 6 ) and T20 ( n = 6 ) group: 50 μL 2 ％ osthole was administered epidurally on the 2th, 6th, 13th day and 20th after surgery respectively. General behaviors were observed and 50％ paw withdrawal threshold(50％ PWT) was measured 1 day before surgery, on the 1 st,3th,7th, 14th,21 th,28th day after surgery, immediately before and 1 hour after osthole or tween-80 administration in each group. On the 7th day after surgery,the left L5 DRGs were obtained for detecting the expression of NOS and COX-2 in M,C and T6 group with 6 rats. Results: No lameness or autophagy was oberserved. 50％ PWT decreased after surgery ( P ＜ 0. 05 ). In T2 and T6 group,50％ PWT after osthole administration were significantly higher than those of M group and C group (P ＜0. 05 ) ,which recovered to the same level as 1 day before surgery (P ＞0. 05). In T13 and T20 group,50％ PWT 1 hour after osthole administration were significantly higher than those of M group and C group ( P ＜ 0. 05 ), which recovered to the same level as 1 day before surgery ( P ＞ 0. 05 ),but on days after 1 hour after administration, there was no significant difference when 50％ PWT compared with M group or nucleus C group (P ＞0. 05 ). NOS positive cells and COX-2 positive cells were no significant difference when M group compared with C group (P＞0.05). But these positive cells in T6 group were significantly lower than those of M group and C group (P ＜0. 05). Conclusion: 50 μL 2％ osthole could completely inhibit the mechanical allodynia in the rat model of sciatica induced by lumbar disc herniation when it was administered epidurally on 2 or 6 day after surgery. But when administered on 13 or 20 day after surgery,its analgesic effect was transient. The effect of 50μ L 2％ osthole epidural administration on day 6 after surgery on the rat model of sciatica induced by lumbar disc herniation may relate to inhibition of the expression of COX-2 and NOS in DRG.关键词
蛇床子素/髓核/坐骨神经痛/一氧化氮合酶/环氧合酶-2Key words
Osthole/ Nucleus pulposus/ Sciatica/ Nitric oxide synsase/ Cyclooxygenase-2分类
医药卫生引用本文复制引用
魏明,张劲军,贺秋兰,王磊,任政华,孙来保,刘先国..蛇床子素对腰椎间盘突出致坐骨神经痛大鼠的作用及其机制研究[J].中药材,2011,34(5):746-750,5.基金项目
中山大学医院与生命科学院合作基金(162011) （162011）
广东省自然科学基金(8161008901000194) （8161008901000194）
