中药材2011，Vol.34Issue(5)：782-786,5.
响应面法优化羊肚菌胞外多糖的发酵条件
Optimization of Culture Conditions for Production of Extracellular Polysaccharides by Morchella esculenta
摘要
Abstract
Objective: To optimize the conditions for exopolysaccharides (EPS)production by Morchella esculenta GIM 5. 69.Methods: A Plackett-Burman design was used to evaluate the effects of variables factors The optimal combined conditions for maximum polysaccharide production were further optimized by response surface methodology. Results:The optimal fermentation conditions were determined as follows:glucose 30％ ,cultivation time 5.98 d,rotary speed 217.44 r/min. The average yield of polysaccharide validation experiments was 53.04％, the relative error was 1.64％. Conclusion: This research has the vital significance regarding the Morchella esculenta polysaccharide function of the product devolopment and the application.关键词
羊肚菌/胞外多糖/Plackett-Burman/Box-Behnken/响应面法Key words
Morchella esculenta/ Extracellular polysaccharides/ Plackett-Burman/ Box-Behnken/ Response surface methodology分类
医药卫生引用本文复制引用
崔华丽,刘增武,方玉明,开桂青,陈彦..响应面法优化羊肚菌胞外多糖的发酵条件[J].中药材,2011,34(5):782-786,5.基金项目
安徽省教育厅重点科研项目(KJ200A172) （KJ200A172）
