基础医学与临床2011，Vol.31Issue(7)：820-826,7.
构建海人酸诱导的内侧颞叶癫痫小鼠模型
Establishment of kainic acid-induced mice models of medial temporal lobe epilepsy
摘要
Abstract
Objective To establish the kainic acid (KA)-induced mesial temporal lobe epilepsy (TLE) mice model and detect the characteristic changes. Methods Healthy male C57BL/6 mice were randomly divided into control group (non-injected), saline group (35 μL/g) and KA group (12 mg/kg) for intrahippocampal injections. 5 days and 5 weeks after injection,the mice brains were sectioned and stained by hemotoxylin-eosin (H&E) to detect the pathologic changes in the hippocampal area; the expression level of P-S6 protein, the marker of hippocampus mTOR signal pathway, was also determinated to verify the animal model. Results (1)Only KA-treated mice showed wet dog shakes, facial clonus and generalized tonic-clonic convulsions. (2)After injection of KA, neuronal cell loss was prominent in in CA1, CA3 and hilar area of the ipsilateral hippocampus; granule cell in dentate gyrus area dispersed, which are fertures of hippocampus sclerosis. ( 3 ) P-S6 expression level increased in both acute ( P ＜ 0. 05 ) and chronic phases (P ＜ 0. 01 ) of MTLE models. Conclusion Intrahippocampal injection of KA in C57BL/6 mice could induce behavioural, histopathological changes which are similar to those clinic features of MTLE.关键词
内侧颞叶癫痫/海人酸/C57BL/6小鼠/海马硬化Key words
mesial temporal lobe epilepsy/ kainic acid/ C57BL/6 mice/ hippocampus sclerosis分类
医药卫生引用本文复制引用
何婷婷,张丹,幸小亮,沙龙泽,金丽日,沈岩,吴立文,许琪..构建海人酸诱导的内侧颞叶癫痫小鼠模型[J].基础医学与临床,2011,31(7):820-826,7.基金项目
国家自然科学基金(30971001,30721063) （30971001,30721063）
北京自然科学基金(7102109) （7102109）
霍英东教育基金(121024) （121024）
