摘要
Abstract
Objective To determine the expression profiles of PTPRO and P53 in human gastric carcinoma tissue, and analyze the relationship of the expressions with clinicopathological characteristics and prognosis of patients with gastric carcinoma. Methods The clinical data of 61 patients with gastric carcinoma of stage Ⅱ-ⅢC. admitted to General Hospital of PLA from Jan. 2004 to Dec. 2007,having undergone D2 radical gastrectomy followed bv 5-FU-based adjuvant chemotherapy, were retrospectively analyzed.Immunohistochemistry was employed to examine the PTPRO expression in samples of primary tumor and paired normal tissue thereof, and lymph nodes with metastasis. The P53 expression in samples of primary tumor was also detected. The relation of P53 and PTPRO expressions to clinicopathological characteristics and prognosis of patients were analyzed. Results In 61 samples, PTPRO was expressed in primary tumor, paired normal tissue and lymph nodes with metastasis with a positive rate of 65.6％, 94.8％ and 65.9％, respectively (P＜0.01). P53 was expressed in primary tumor with a positive rate of 36.2％. No correlation existed between p53 expression and PTPRO expression ( r=0.169, P=0.20) . Kaplan-Meier survival analysis showed that the median disease-free survival time (MDFS) was longer in patients with negative P53 expression (P=0.022) and early stage carcinoma ( P=0.000), and the median survival time (MST)was also longer in patients with negative P53 expression ( P=0.001) and early stage carcinoma ( P=0.001). Cox regression analysis showed that the clinical stage (P=0.001) , PTPRO and P53 expressions (P=0.018, P=0.031) were the independent prognostic factors for disease-free survival (DFS), and clinical stage ( P=0.05), PTPRO and P53 expressions ( P=0.014, P=0.033) and pathological stage (P=0.020) were independent prognostic factors for over survival (OS). Conclusions The expression of PTPRO is notably decreased in gastric carcinoma. The expression of PTPRO and P53 proteins are expected to be molecular markers for predicting the prognosis of patients with gastric carcinoma who received 5-FU-based adjuvant chemotherapy after D2 radical gastrectomy.关键词
胃肿瘤/PTPRO蛋白,人/肿瘤抑制蛋白质p53/无病生存/Kaplan-Meiers评估Key words
stomach neoplasms/ PTPRO protein, human/ tumor suppressor protein p53/ disease-free survival/ Kaplan-Meiers estimate分类
医药卫生
